International Journal of Infection Control International Journal of Infection Control International Federation of Infection Control (IFIC) en-US International Journal of Infection Control 1996-9783 <p><span style="color: #4b7d92;">Authors retain copyright of their work, with first publication rights granted to IJIC. Read the full <a href="">Copyright- and Licensing Statement</a>.</span></p> Effect of environmental and behavioural factors on microbiological air quality of operating rooms <p><strong><em>Introduction:</em></strong>&nbsp;An operating theatre (OT) is a very complex setup, which presents numerous challenges for both patients and health care providers. A safe OT environment decreases the susceptibility of patients to postoperative infections. Therefore, a study was conducted to determine microbiological air quality for establishing baseline values and to assess concurrently the effect of environment and behavioural factors in 46 OTs of a tertiary care hospital.</p> <p><strong><em>Materials &amp; Methods:</em></strong>&nbsp;The OTs were divided into two groups based on the type of air flow: laminar diffuser (Group I) or conventional diffuser (Group II). Two hundred and thirty-eight samples were collected with an active technique using a sieve impactor, sampling at 100 L/minute. Statistical analysis was conducted using Statistical Package for Social Sciences (SPSS) Version 20 (IBM Corp., Armonk, NY).</p> <p><strong><em>Results:</em></strong>&nbsp;In Group I OTs with the laminar diffuser, bacterial and fungal values during the lean period for environmental and behavioural factors were 47.78 colony forming units (CFM)/m<sup>3</sup>&nbsp;and 0.24 CFU/m<sup>3</sup>, respectively. The bacterial and fungal values during the surgical period were 98.01 CFU/m3 and 0.40 CFU/m<sup>3</sup>, respectively. The same values for Group II OTs with the conventional diffuser were 52.83 CFU/m<sup>3</sup>&nbsp;and 0.39 CFU/m<sup>3</sup>&nbsp;during the lean period, and 80.06 CFU/m<sup>3</sup>&nbsp;and 0.40 CFU/m<sup>3</sup>&nbsp;during the surgical period.</p> <p><strong><em>Discussion:</em></strong>&nbsp;Both environmental and behavioural factors – temperature, humidity, percentage of fresh air in circulation, door type, controlled entry, minimal door opening and proper OT attire – were seen to contribute concurrently to maintaining air quality in operating units.</p> Gurmail Singh Manisha Biswal Vipin Koushal Navneet Dhaliwal ##submission.copyrightStatement## 2022-01-19 2022-01-19 18 10.3396/ijic.v18.21204